Complementary recognition in condensed DNA: Accelerated DNA renaturation
نویسندگان
چکیده
منابع مشابه
Complementary recognition in condensed DNA: accelerated DNA renaturation.
The functional consequences of DNA condensation are investigated. The recognition of complementary strands is profoundly modified by this critical phenomenon. (1) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including polymers, di- and multivalent cations, as we...
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The rate of renaturation of fully denatured DNA is kinetically a second-order reaction. The reaction rate increases as the temperature decreases below T,t, reaching a broad flat maximum from 15 to 30°C below T, and then decreases with a further decrease in temperature. Let N be the complexity of the DNA or the number of base-pairs iu non-repeating sequences per virus or cell for the given DNA, ...
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OBJECTIVES The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was de...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 1991
ISSN: 0022-2836
DOI: 10.1016/0022-2836(91)90595-w